Vitamin D test  

The Vitamin D rapid test is a rapid chromatographic immunoassay for the semi-quantitative detection of 25-hydroxyvitamin D (25 (OH) D) in human fingerstick whole blood For self-testing in vitro diagnostic use.  This assay provides a preliminary diagnostic test result and can be used to screen for Vitamin D deficiency.  

Vitamin D refers to a group of fat-soluble secosteroids responsible for increasing intestinal absorption of calcium, iron, magnesium, phosphate and zinc. In humans, the most important compounds in this group are vitamin D3 and vitamin D2 

Vitamin D3 is naturally produced in the human skin through the exposure to ultraviolet light and Vitamin D2 is mainly obtained from foods. Vitamin D is transported to the liver where it is metabolised to 25-hydroxy Vitamin D. In medicine, a 25-hydroxy Vitamin D blood test is used to determine Vitamin D concentration in the body. The blood concentration of 25-hydroxy Vitamin D (including D2 and D3) is considered the best indicator of Vitamin D status. Vitamin D deficiency is now recognised as a global epidemic. 




Our tests will not be accepted for travel to Hong Kong.
Our tests will not be accepted for travel to Hong Kong.

Virtually every cell in our body has receptors for Vitamin D, meaning that they all require “sufficient” level of Vitamin D for adequate functioning. The health risks associated with Vitamin D deficiency are far more severe than previously thought. Vitamin deficiency has been linked to various serious diseases: osteoporosis, osteomalacia, multiple sclerosis, cardiovascular diseases, pregnancy complications, diabetes, depression, strokes, autoimmune diseases, flu, different cancers, infectious diseases, alzheimer, obesity and higher mortality etc. 

The Vitamin D test is an immunoassay based on the principle of competitive binding. During testing, the mixture migrates upward on the membrane chromatographically by capillary action. The membrane is pre-coated with 25 (OH) D antigens on the test line region of the strip. During testing, 25 (OH) D present in the specimen will compete with 25 (OH) D on the test line for a limited amount of anti-25 OH Vitamin D antibodies in the conjugate. The higher concentration of 25 (OH) D in the specimen, the lighter would be the T line. The result will be read according to the Colour card provided with the kit. To serve as a procedural control, a coloured line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred. 

Please ensure that you read the instructions for use before taking this test  


If your test is positive it is highly likely that you are vitamin D deficient. It is important that you seek advice from your own GP in order to have a full blood test taken to establish exactly how low your level is. This will help inform the best treatment for you. Presently, you can purchase Vitamin D tablets from your local pharmacy or supermarket. You should ensure you purchase a minimum dosage of 800iu.  

If your test is negative it is highly likely that your Vitamin D levels are sufficient. You do not need to access any further treatment. 


Belfast Test Centre

7 Lower Crescent Belfast Co Antrim BT7 1NR

Liverpool Rodney Street

57 Rodney Street, Liverpool, L1 9AT

Bank Test Centre

32 Cornhill, London EC3V 3SG, UK

Manchester Test Centre

Unit 3, Empress Business Centre Chester Rd M16 9EA

Wirral Test Centre

Skinplicity, 9 Church Road, Bebington, CH63 7PG​.

Manchester Test Centre

Unit 3, Empress Business Centre Chester Rd M16 9EA

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